paired end sequencing read length

Web Coverage read depth or depth is the average number of reads representing a given nucleotide in the reconstructed sequence. It can be calculated from the length of the original genome G the number of readsN and the average read lengthL as For example a hypothetical genome with 2000 base pairs reconstructed from 8 reads with an average.


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Reads of length 20-40 base pairs bp are referred to as ultra-short.

. Read length is a factor which can affect the results of biological. Typical sequencers produce read lengths in the range of 100-500 bp. However Pacific Biosciences platforms produce read lengths of approximately 1500 bp.

Sequencing technologies vary in the length of reads produced.


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